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ARS Liquid Semen Extenders are designed to be general purpose stallion semen extenders used with fresh semen for immediate insemination or for semen that will be cooled and stored or transported to another location prior to insemination of the mare. All three ARS Semen Extender formulas were developed and evaluated by the Colorado State University Equine Reproduction Laboratory (ERL).

All ARS extenders rely on a two-part packaging system to ensure freshness of the antibiotic. Most antibiotics lose effectiveness over time when stored in a liquid form. ARS ensures the effectiveness of our antibiotics by storing the ingredients in a stable dry form until mixing just before the extender is added to the stallion semen.

ARS BLUE™ is a new advanced extender specially formulated to maximize fertility in semen that has been cooled and or transported. The original E-Z Mixin® family of extenders have been the industry standard for many years. These Kenney-based formulas have been adjusted to allow the use of several different antibiotics to prevent bacterial growth and to optimize the length of time the semen can be cooled and stored prior to use. Finally, the E-Z Mixin® 10 family of extenders are a non-Kenney based formula that has been shown to work well in stallions whose semen does not cool well with a traditional Kenney extender.

ARS Semen Extenders

Antibiotic	ARS BLUE	E-Z Mixin	E-Z Mixin 10
Amikacin	ARS BLUE with Amikacin Casein-based extender that can be used for fresh insemination or cooled storage. This extender can be used as a routine multi-purpose extender (i.e. fresh or cooled semen) and can also be used for stallions that do not cool well in a traditional Kenney-based extender. Visit Product Page	E-Z Mixin - “CST” Excellent Kenney-based extender formulation for general use. The basic formula contains the antibiotic amikacin. Can be used for extending stallion semen for fresh insemination or for cooled storage and transport. Visit Product Page	E-Z Mixin 10-“CST” A non-Kenney based formulation for fresh insemination or cooled storage. This extender may be beneficial for stallion semen that does not cool well in a traditional Kenney-based extender. The basic E-Z Mixin 10 extender contains the antibiotic amikacin. Visit Product Page
Amikacin plus Potassium Penicillin G	ARS BLUE with Amikacin and K Pen G Casein-based extender that can be used for fresh insemination or cooled storage. The addition of potassium penicillin G provides a broader spectrum of bacterial growth control than amikacin alone. Visit Product Page	E-Z Mixin - “CST+” Antibiotic option for stallions that cool well in a Kenney-based formulation. The addition of potassium penicillin G provides a broader spectrum of bacterial growth control than amikacin alone. Visit Product Page	E-Z Mixin 10-“CST+” A non-Kenney based formulation for fresh insemination or cooled storage. The addition of potassium penicillin G provides a broader spectrum of bacterial growth control than amikacin alone. Visit Product Page
Ticarcillin	ARS BLUE Basic Formula with Ticarcillin Casein-based extender that can be used for fresh insemination or cooled storage. Ticarcillin provides an alternative antibiotic to amikacin (± penicillin) and will provide broad-spectrum control of bacterial growth. Visit Product Page	E-Z Mixin - “BFT” Antibiotic option for stallions that cool well in a Kenney-based formulation, but require a different antibiotic than amikacin to maintain optimal sperm motility with cooled storage. Ticarcillin will provide broad-spectrum control of bacterial growth. Visit Product Page	E-Z Mixin 10-“BFT” A non-Kenney based formulation for fresh insemination or cooled storage. ticarcillin provides an alternative antibiotic to amikacin (± penicillin) and will provide broad-spectrum control of bacterial growth. Visit Product Page
Potassium Penicillin G	ARS BLUE Basic Formula with K Pen G Casein-based extender that can be used for fresh insemination or cooled storage. potassium penicillin G will primarily provide control of growth of Gram-positive organisms. Visit Product Page	E-Z Mixin - “BF+” E-Z Mixin Antibiotic option for stallions that cool well in a Kenney-based formulation, but require a different antibiotic than amikacin to maintain optimal sperm motility with cooled storage. Potassium penicillin G will primarily provide control of growth of Gram-positive organisms. Visit Product Page	E-Z Mixin 10- “BF+” A non-Kenney based formulation for fresh insemination or cooled storage. Potassium penicillin G will primarily provide control of growth of Gram-positive organisms. Visit Product Page
None (Basic Formula; “BF”)	ARS Blue Basic Formula An alternate general purpose centrifugation media. Casein-based extender for use when custom antibiotics are required to treat a specific condition. Visit Product Page	E-Z Mixin - “BF” A good general purpose centrifugation media. Kenney-based extender for use when custom antibiotics are required to treat a specific condition. Visit Product Page	E-Z Mixin 10 -“BF” A good general purpose centrifugation media. Non-Kenney-based extender for use when custom antibiotics are required to treat a specific condition. Visit Product Page

Antibiotic selection is one of the most important decisions when choosing any extender. Colorado State University (ERL) and Animal Reproduction Systems both strongly recommend the use of an extender containing an appropriate antibiotic for any stallion semen used for insemination. Amikacin is a good general purpose antibiotic that is tolerated by most stallion spermatozoa. Consequently, this is the antibiotic of choice for most stallions. Ticarcillin is a good alternative for stallion semen that does not cool well in the presence of amikacin. In some situations, the addition of potassium penicillin G to an extender containing amikacin can provide additional broad spectrum protection to the standard amikacin based extender.

Each ARS formula has a “BF” or basic formula version that can be used as a centrifugation medium or as a base extender when custom antibiotics are required.

HOW TO SELECT AN ANTIBIOTIC